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Part:BBa_K1524107

Designed by: Hana SHIMOYAMA   Group: iGEM14_HokkaidoU_Japan   (2014-10-11)

anti-sense B0034

Anti-sense B0034 silence any protein controlled RBS, B0034. Anti-sense B0034 uses H-stem vector (K1524100). It is induced by adding IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 319
    Illegal PstI site found at 468
    Illegal NotI site found at 461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 239


[edit]
Categories
Parameters
None